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Expand your T cell research with confidence
The BD Horizon™ Human T Cell Backbone Panel consists of an optimized configuration of core markers specifically designed to expand your research with recommended fluorochrome drop-ins and enable flexibility in panel design without compromising resolution.
Fluorochromes were strategically selected so that up to five recommended drop-ins can be added with minimal impact to the backbone resolution and minimal panel design effort, allowing you to focus on marker selection.
Learn more from the BD Horizon™ Human T cell backbone panel brochure.
Watch the webinar describing the use of the BD Horizon™ T Cell Backbone Panel for expanding your T cell research.
The BD Horizon™ Human T Cell Backbone Panel contains a set of markers that can be combined with up to five additional fluorochromes without impacting resolution
| Marker | Clone | Fluorochrome |
|---|---|---|
| CD3 | UCHT1 | BV510 |
| CD4 | SK3 | BV786 |
| CD8 | RPA-T8 | R718 |
| CD45RA | HI100 | PE-Cy7 |
| CD197 (CCR7) | 2-L1-A | BV711 |
BD Horizon™ Brilliant Stain Buffer is included to provide optimal reagent performance.
Examples of recommended drop-in fluorochromes
| Laser | Drop-in |
|---|---|
| Violet 405 nm | BV421/V450/Pacific Blue™ |
| Blue 488 nm | FITC/BB515/Alexa Fluor™ 488/PE |
| Yellow-Green 561 nm | PE/RY586 |
| Red 640 nm | APC/Alexa Fluor™ 647 |
| UV 355 nm | BUV395 |
Drop-in reagents not included in backbone kit.
AF: Alexa Fluor™ Reagent
BB: BD Horizon Brilliant™ Blue Reagent, BUV: BD Horizon Brilliant™ Ultraviolet Reagent
BV: BD Horizon Brilliant™ Violet Reagent, R: BD Horizon™ Red Reagent
RY: BD Horizon RealYellow™ Reagent
The BD Horizon RealYellow™ (RY) 586 Fluorochrome can be used as an alternative to PE when excited off the yellow-green laser. Simultaneous use of PE off the blue laser and RY586 off the yellow-green laser is not recommended.
The BD Horizon™ Human T Cell Backbone Panel offers increased flexibility and fewer design challenges
High Performance
- Strategically designed to be complemented with up to five drop-in markers with no resolution impact to the backbone or the selected drop-ins
- Verified protocols to ensure optimal performance
- Consistent performance across instruments with different optical configurations
Performance
Performance of BD HorizonTM Human T Cell Backbone Panel including drop-in configurations
Applications
Identification of FoxP3+ Treg subsets
A) The backbone panel was complemented with surface and intracellular markers conventionally used to identify human Treg subsets.
B) Clear resolution of all the subsets of interest was observed on fixed and permeabilized PMBCs from N = 3 healthy donors. Samples were acquired on a 4-laser BD FACSymphony™ A1 Cell Analyzer and analyzed with FlowJo™ Software v10.8.1.
B) Clear resolution of all the subsets of interest was observed on fixed and permeabilized PMBCs from N = 3 healthy donors. Samples were acquired on a 4-laser BD FACSymphony™ A1 Cell Analyzer and analyzed with FlowJo™ Software v10.8.1.
Upregulation of inhibitory receptors
BD Horizon™ Human T Cell Backbone Panel Kit
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BD flow cytometers are Class 1 Laser Products.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Alexa Fluor and Pacific Blue are trademarks of Thermo Fisher Scientific. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.