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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
This antibody was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with rat, human, bovine, and horse serum proteins (multiple adsorption).
Recommended Assay Procedures
Flow cytometry: As a second step reagent for staining rat splenocytes, it is reactive with mouse antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. However it has been reported that reactivity with some mouse IgM monoclonal antibodies is weak. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with no non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse antibodies, particularly mouse IgG. For optimal staining of human leukocytes, with mouse IgG and IgM primary antibodies, FITC goat anti-mouse IgG + IgM (human-, bovine-, and horse-adsorbed, Cat. No. 555988) is recommended.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
No Citations are available for this Product
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.