BD Transduction Laboratories™ Purified Mouse Anti-PKA[RIIα]
Clone 40/PKA[RIIα] (RUO)
Western blot analysis of PKA[RIIα] on K562 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of PKA[RIIα].
Immunofluorescence staining of HeLa cells.
Western blot analysis of PKA[RIIα] on K562 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of PKA[RIIα].
Immunofluorescence staining of HeLa cells.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic(C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIß, RIIα, and RIIß.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cß, or Cγ). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Rα isoforms are ubiquitously expressed, the Rß isoforms are predominant in nervous and adipose tissues. Expression of the RIα subunit is modulated during muscle and adipocyte differentiation in vitro.
Development References (5)
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Fukuyama T, Sueoka E, Sugio Y, et al. MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes. Oncogene. 2001; 20(43):6225-6232. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Sandberg M, Skalhegg B, Jahnsen T. The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals. Biochem Biophys Res Commun. 1990; 167(1):323-330. (Biology). View Reference
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Takahashi M, Mukai H, Oishi K, Isagawa T, Ono Y. Association of immature hypophosphorylated protein kinase cepsilon with an anchoring protein CG-NAP. J Biol Chem. 2000; 275(44):34592-34596. (Clone-specific: Western blot). View Reference
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Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A. A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta. J Biol Chem. 2002; 277(40):36955-36961. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Westphal RS, Soderling SH, Alto NM, Langeberg LK, Scott JD. Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold. EMBO J. 2000; 19(17):4589-4600. (Clone-specific: Immunofluorescence). View Reference
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