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      Purified Mouse Anti-Phosphoserine/threonine

      BD Transduction Laboratories™ Purified Mouse Anti-Phosphoserine/threonine

      Clone 22A/pSer/Thr (RUO)

      View all Formats
      S38820_612549_image4.png

      Immunofluorescence staining of phosphoserine/threonine (clone 22A; MN 612548) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.

      S38820_612549_image3.png

      Immunofluorescence staining of phosphoserine/threonine (clone 22A; MN 612548) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      S38820_612549_image1.png

      Immunofluorescence staining of phosphoserine (clone 19; MN 612546) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      S38820_612549_image2.png

      Immunofluorescence staining of phosphoserine (clone 19; MN 612546) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.

      S38820_612549_image4.png S38820_612549_image3.png S38820_612549_image1.png S38820_612549_image2.png Blue-Cap-White-Label.jpg

      Immunofluorescence staining of phosphoserine/threonine (clone 22A; MN 612548) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.

      Immunofluorescence staining of phosphoserine/threonine (clone 22A; MN 612548) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      Immunofluorescence staining of phosphoserine (clone 19; MN 612546) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

      Immunofluorescence staining of phosphoserine (clone 19; MN 612546) on A431 cells (Human epithelial carcinoma; ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.

      Product Details
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      BD Transduction Laboratories™
      Human (QC Testing), Rat (Tested in Development)
      Mouse IgG1
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      250 µg/ml
      AB_399844
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

      For western blotting, a dilution of 1:2500 may be a useful starting concentration for titrations.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      612548 Rev. 1
      Antibody Details
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      22A/pSer/Thr

      Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events.  An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response.  Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine.

      612548 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      612548 Rev.1
      Citations & References
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      View product citations for antibody "612548" on CiteAb

      Development References (3)

      1. Brivanlou AH, Darnell JE Jr. Signal transduction and the control of gene expression. Science. 2002; 295(5556):813-818. (Biology). View Reference
      2. Pawson T, Scott JD. Signaling through scaffold, anchoring, and adaptor proteins. Science. 1997; 278(5346):2075-2080. (Biology). View Reference
      3. Yan JX, Packer NH, Gooley AA, Williams KL. Protein phosphorylation: technologies for the identification of phosphoamino acids. J Chromatogr A. 1998; 808(1-2):23-41. (Biology). View Reference
      612548 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.