BD Transduction Laboratories™ Purified Mouse Anti-Flotillin-1
Clone 18/Flotillin-1 (RUO)
Western blot analysis of flotillin-1 on rat brain lysate. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of flotillin-1.
Western blot analysis of flotillin-1 on rat brain lysate. Lane 1: 1:250, Lane 2: 1:500, Lane 3: 1:1000 dilution of flotillin-1.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caveolae are specialized membrane invaginations of 50-100 nm present in all cells, but abundant in endothelium, muscle cells, and adipocytes. These plasma membrane microdomains function in transcytosis of macromolecules, and are the sites of potocytosis, where small molecules are concentrated and transferred inside the cells by glycosylphosphatidylinositol (GPI)-linked receptors. Caveolin, a 22kDa protein and a well known marker for these plasma membrane microdomains, plays a structural role in these specializations. Flotillin-1 was isolated from the Triton X-100 insoluble buoyant fraction, characteristic of caveolae. Although the mRNA expression of both Flotillin-1 and Caveolin is very similar, Caveolin is undetectable in brain, while Flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/Flotillin-2), which also colocalizes in the caveolae. Thus, Flotillin-1 and its relative ESA/Flotillin-2 are now incorporated into the expanding list of proteins co-localized at the caveolae which includes PKCα, Ras, Rap Src-like kinases, Gαßγ,and GPI-linked receptors.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem. 1997; 272(21):13793-13802. (Biology). View Reference
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Cabin DE, Shimazu K, Murphy D, et al. Synaptic vesicle depletion correlates with attenuated synaptic responses to prolonged repetitive stimulation in mice lacking alpha-synuclein. J Neurosci. 2002; 22(20):8797-8807. (Clone-specific: Western blot). View Reference
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Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA. Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain. J Biol Chem. 2002; 277(1):486-491. (Clone-specific: Western blot). View Reference
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Morrow IC, Rea S, Martin S, et al. Flotillin-1/reggie-2 traffics to surface raft domains via a novel golgi-independent pathway. Identification of a novel membrane targeting domain and a role for palmitoylation. J Biol Chem. 2002; 277(50):48834-48841. (Clone-specific: Immunofluorescence). View Reference
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Rybin VO, Xu X, Lisanti MP, Steinberg SF. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 2000; 275(52):41447-41457. (Clone-specific: Electron microscopy, Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.