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      Purified Mouse Anti-CASK
      Product Details
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      BD Transduction Laboratories™
      Rat (QC Testing), Human, Mouse, Dog, Frog (Tested in Development)
      Mouse IgG1
      Rat CASK aa. 353-486
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
      120 kDa
      250 µg/ml
      AB_398103
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610782 Rev. 1
      Antibody Details
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      7/CASK

      CASK is a recently identified cytosolic protein kinase with homology to the Ca2+/CaM-dependent kinases and the synaptic associated proteins SAPs/PSDs. Like the SAPs, CASK contains a PDZ domain, an SH3 region, and a guanylate kinase domain. However, unlike the rest of the PDZ protein family, the amino terminus of CASK has significant homology with the Ca2+/Calmodulin-dependent kinases. Although widely expressed, CASK is highly enriched in the synaptic plasma membrane where it associates with neurexins, the neuronal cell surface proteins. Neurexins are a complex family of surface proteins that act as receptors for a number of venoms and toxins and regulate the clustering of several ion channels at the synapse. In addition, neurexins bind heterotypically to neuroligins, therefore adjoining different cell types. Neuroligins bind intracellularly to PSD95 and related proteins, whereas neurexins bind to CASK through their C-terminal region and at CASK's PDZ domain. The interaction of neurexins and CASK at the outside of the cell may modulate CASK's activity and trigger an intracellular signaling cascade.

      610782 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610782 Rev.1
      Citations & References
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      View product citations for antibody "610782" on CiteAb

      Development References (5)

      1. Biederer T, Sudhof TC. CASK and protein 4.1 support F-actin nucleation on neurexins. J Biochem (Tokyo). 2001; 276(51):47869-47876. (Biology: Western blot). View Reference
      2. Borg JP, Lõpez-Figueroa MO, de Taddèo-Borg M. Molecular analysis of the X11-mLin-2/CASK complex in brain. J Neurosci. 1999; 19(4):1307-1316. (Biology: Western blot). View Reference
      3. Fallon L, Moreau F, Croft BG, Labib N, Gu WJ, Fon EA. Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain. J Biol Chem. 2002; 277(1):486-491. (Biology: Immunofluorescence, Western blot). View Reference
      4. Hata Y, Butz S, Südhof TC. CASK: a novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins. J Neurosci. 1996; 16(8):2488-2494. (Biology). View Reference
      5. Irie M, Hata Y, Takeuchi M. Binding of neuroligins to PSD-95. Science. 1997; 277(5331):1511-1515. (Biology). View Reference
      View All (5) View Less
      610782 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.