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Game 6: Monocyte Background
Welcome to the BD Biosciences Fluorochrome Faceoff, where we explore the performance of various flow cytometry dyes excited primarily by the blue and yellow-green lasers!
This game will evaluate fluorochromes based on monocyte background staining.
What is Monocyte Background?
It has been shown1 that some cyanine (Cy™)—and cyanine-like—dyes can nonspecifically bind to monocytes. This interaction is thought to be, at least in part, due to binding to the human Fc receptor. Nonspecific monocyte binding can decrease data resolution by increasing background and/or causing a false positive signal.
In this Fluorochrome Faceoff, we will evaluate dyes excited by the yellow-green laser with peak emission around 780 nm on several clones.
1. Shapiro HM. Practical Flow Cytometry. 4th ed. Hoboken: John Wiley & Sons, Inc., 2005.
Who Do You Think Will Win?
Competitors | |
BD Horizon RealYellow™ 775 (RY775) | BD Pharmingen™ PE-Cy7 |
Bio-Rad StarBright™ Yellow 800 (SBY800) | BioLegend PE/Fire™ 810 |
*Note: Poll results do not determine the winners. Come back on September 26 to see game results and winners based on fluorochrome performance data.
Current Poll Standings as of September 12, 2024
RY775: 73% | PE/Fire™ 810: 17% | PE-Cy7: 6% | SBY800: 4%
The Results will be Announced Thursday, September 26
Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.
BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.