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Real Yellow/Real Blue Family Campaign

For Professionals in Research

BD Horizon RealYellow™ Reagents and RealBlue™ Reagents

The superior alternative to PerCP and PE tandem dyes

 

Reduced Monocyte Background

Reduced Monocyte 
Background

Reduced Spillover

Intracellular Staining

reduced monocyte background

BD Horizon RealYellow Reagents and RealBlueReagents Offer Decreased Monocyte Background Compared to PE-Cy Tandems

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BD HorizonRB780 Reagents offer reduced background compared to PE-Cy7

RY775-Improved-nonspecific-Binding
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BD Horizon™ RY775 Reagents provide improved nonspecific binding

reduced spillover

BD Horizon RealYellow Reagents and RealBlue Reagents Offer Reduced Spillover Compared to PE and PerCP Tandems

RB610 Reduced Spillover Compared to PE Dazzle and PE CF594
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BD Horizon RY610 Reagents show reduced spillover into the corresponding blue detectors.

RB705 Reduced Spillover Compared with PerCP and CY5.5
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BD Horizon RB705 Reagents show reduced spillover into the corresponding yellow-green detectors.

RY586 Reduced Spillover Compared with PE Tandem Dyes
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BD Horizon RY586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BDFACSymphony A5 SE Cell Analyser.

Intracellular marker detection

BD Horizon RealYellow and RealBlue Reagents Offer the Detection of Low-expression Surface and Intracellular Markers.

RY703-Reagent-Lower-Expression-Intracellular-Markers
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BD Horizon RY703 Reagent resolves low-expression intracellular markers

Cytokine-Marker
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BD Horizon RB780 Reagents support the detection of low-expression intracellular markers.

RY775-Resolves-Intracellular-Markers
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BD Horizon RY775 Reagent resolves intracellular markers

 

 

Swap Your Fluorochromes for the Cleaner Versions

FormatSpectralConventionalRelative BrightnessSpillover
(1-low, 4-high)
Alternative To
Blue Laser
RB545  1(Unique position)
RB613 2PE-CF594, PE-Dazzle™ 594, BB630-P2 or StarBright™ Blue 615
RB670 2PE-Cy5, BB660-P2 or StarBright™ Blue 675
RB705 2PerCP-Cy5.5, BB700 or StarBright™ Blue 700
RB744 1BB755-P
RB780 1PE-Cy7 or BB790-P
Yellow-Green Laser
RY586 1PE
RY610 1PE-CF594 or PE-Dazzle™ 594
RY655 3PE-Cy5 or StarBright Yellow 665
RY703 2PE-Cy5.5 or StarBright™ Yellow 720
RY743 2PE/Fire™ 744
RY775 2PE-Cy7 or StarBright™ Yellow 800
* On three-laser configuration (B, V, R)
** On four- or five-laser configuration (B, V, R, YG or UV, V, B, YG, R)

 

For more details on these fluorochromes, visit the BD Horizon RealYellow™ Reagents and BD Horizon RealBlue™ Reagents page.

 

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Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.