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This is a suggested alternate SKU for 551052.

BD Pharmingen™ Purified Hamster Anti-Human Bcl-2

Clone 6C8 (RUO)

1513GN_551051_image1.png

Western blot analysis of Bcl-2.  A Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with the hamster anti-human Bcl-2 antibody at concentrations of 3.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3).  Bcl-2 can be identified as a band of ~ 26 kDa.

1513GN_551051_image1.png 551024_01.png Red Cap_UV.png Orange-Cap_UV.png Blue-Cap-Red-Label.jpg

Western blot analysis of Bcl-2.  A Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with the hamster anti-human Bcl-2 antibody at concentrations of 3.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3).  Bcl-2 can be identified as a band of ~ 26 kDa.

Product Details
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BD Pharmingen™
Human (QC Testing)
Western blot (Routinely Tested), Flow cytometry, Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
RUO


Description

Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Store both the hamster anti-human Bcl-2 antibody (component 51-1513GR) and the Jurkat cell lysate (component 51-16526N) undiluted at -20°C.

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Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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551051 Rev. 3
Components
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Description Quantity/Size Part Number Clone Isotype
Purified Hamster Anti-Human Bcl-2 50 µg (1 ea) 51-1513GR 6C8 IgG2, κ
Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A N/A
551051 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.