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Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG2b
Fli-1 ets Domain Fusion Protein
Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Reported)
50 kDa
0.5 mg/ml
AB_395333
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures
G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested at BD Pharmingen.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554266 Rev. 7
Antibody Details
G146-222
Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11;22)(q24;q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.
554266 Rev. 7
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554266 Rev.7
Citations & References
View product citations for antibody "554266" on CiteAb
Development References (2)
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Bailly RA, Bosselut R, Zucman J. DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma. Mol Cell Biol. 1994; 14(5):3230-3241. (Biology). View Reference
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May WA, Lessnick SL, Braun BS. The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1. Mol Cell Biol. 1993; 13(12):7393-7398. (Biology). View Reference
554266 Rev. 7
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.