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BD Transduction Laboratories™ Purified Mouse Anti-p190
Clone 30/p190 (RUO)
Western blot analysis of p190 on HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of p190.
Human Fibroblast
Western blot analysis of p190 on HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of p190.
Human Fibroblast
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Human p190 aa. 1-1513
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
190 kDa
250 µg/ml
AB_397551
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610149 Rev. 1
Antibody Details
30/p190
GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21[ ras]. Ras-GAP (120kDa) is perhaps the best characterized GAP. p190 is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190 are targets of oncoproteins and growth factor receptors. Recombinant p190 enhances GTPase activity of the rho family of GTPases. The complete sequence of p190 exhibits three major regions: an N-terminal region with homology to all GTPases, a middle region homologous to transcriptional repressors, and a C-terminal region the is similar to n-chimerin and other small GTPases.
610149 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610149 Rev.1
Citations & References
View product citations for antibody "610149" on CiteAb
Development References (5)
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Brandt D, Gimona M, Hillmann M, Haller H, Mischak H. Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway. J Biol Chem. 2002; 277(23):20903-20910. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Maddox AS, Burridge K. RhoA is required for cortical retraction and rigidity during mitotic cell rounding. J Cell Biol. 2003; 160(2):255-265. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Nakahara H, Mueller SC, Nomizu M, Yamada Y, Yeh Y, Chen WT. Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia. J Biol Chem. 1998; 273(1):9-12. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Settleman J, Narasimhan V, Foster LC, Weinberg RA. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell. 1992; 69(3):539-549. (Biology). View Reference
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Wolf RM, Draghi N, Liang X. p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human chromosome 19q13.3. Genes Dev. 2003; 17(4):476-487. (Clone-specific: Immunohistochemistry, Western blot). View Reference
610149 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.