Skip to main content Skip to navigation
Purified Mouse Anti-γ-Catenin
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human γ-Catenin aa. 553-738
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
82 kDa
250 µg/ml
AB_397648
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610253 Rev. 1
Antibody Details
Down Arrow Up Arrow
15/γ-Catenin

γ-Catenin (plakoglobin) was identified as a compenent of desmosomes where it associates with desmoglein. γ-Catenin and β-Catenin are closely related proteins that have significant homology with the Drosophila armadillo protein. In addition to complexing with E-Cadherin, γ-Catenin and β-Catenin have been observed in association with the intracellular domain of N-Cadherin. It has been proposed that one molecule of α-Catenin and at least one molecule of β-Catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19 amino acid sequence of desmoglein (Dsg1) was found to be critical for binding of γ-Catenin. This region has significant homology to the catenin-binding domain of classical cadherins, thus suggesting a common mechanism for γ-Catenin localization at both adherens junctions and desmosomes.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610253 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610253 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "610253" on CiteAb

Development References (5)

  1. Mary S, Charrasse S, Meriane M, et al. Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism. Mol Biol Cell. 2002; 13(1):285-301. (Biology: Western blot). View Reference
  2. McCrea PD, Turck CW, Gumbiner B. A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin. Science. 1991; 254(5036):1359-1361. (Biology). View Reference
  3. Merritt AJ, Berika MY, Zhai W, et al. Suprabasal desmoglein 3 expression in the epidermis of transgenic mice results in hyperproliferation and abnormal differentiation. Mol Cell Biol. 2002; 22(16):5846-5858. (Biology: Immunohistochemistry). View Reference
  4. Muller T, Choidas A, Reichmann E, Ullrich A. Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration. J Biol Chem. 1999; 274(15):10173-10183. (Biology: Immunoprecipitation, Western blot). View Reference
  5. Peng YF, Mandai K, Nakanishi H, et al. Restoration of E-cadherin-based cell-cell adhesion by overexpression of nectin in HSC-39 cells, a human signet ring cell gastric cancer cell line. Oncogene. 2002; 21(26):4108-4119. (Biology: Immunofluorescence, Western blot). View Reference
View All (5) View Less
610253 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.