ELISA Capture: The purified BVD10-40F6 antibody (Cat.No. 554493) is useful as a capture antibody for a sandwich ELISA for measuring human IL-7 protein levels. Purified BVD10-40F6 antibody can be paired with the biotinylated BVD10-11C10 antibody (Cat. No. 554494) as the detection antibody, with recombinant human IL-7 (Cat. No. 554608) as the standard. Purified BVD10-40F6 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (eg, mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.
Neutralization: The no azide/low endotoxin format (NA/LE) of BVD10-40F6 antibody, Cat. No. 554492, is recommended for neutralization of human IL-7 bioactivity. A suitable NA/LE rat IgG1 isotype control to match the BVD10-40F6 antibody is the R3-34 antibody, Cat. No, 559072.
Western Blot: The BVD10-40F6 antibody (Cat. No. 554493) has been found useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen