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BD Pharmingen™ Purified Mouse Anti-Itk
Clone 2F12 (RUO)
Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, Cat. No. 550503) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Itk is identified as a band at ~72 kDa.
Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.
Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, Cat. No. 550503) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Itk is identified as a band at ~72 kDa.
Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.
Product Details
BD Pharmingen™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Fusion protein with aa.1-26 of human/mouse Itk
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), In vitro kinase assay (Reported)
72 kDa
0.5 mg/ml
AB_399156
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550503 Rev. 3
Antibody Details
2F12
Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
550503 Rev. 3
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550503 Rev.3
Citations & References
View product citations for antibody "550503" on CiteAb
Development References (3)
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Heyeck SD, Wilcox HM, Bunnell SC, Berg LJ.. Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity.. J Biol Chem. 272(25401)(Immunogen: Immunoprecipitation, In vitro kinase assay, Western blot).
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Liu KQ, Bunnell SC, Gurniak CB, Berg LJ. T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells. J Exp Med. 1998; 187(10):1721-1727. (Clone-specific: Western blot). View Reference
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Siliciano JD, Morrow TA, Desiderio SV. itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2. Proc Natl Acad Sci U S A. 1992; 89(23):11194-11198. (Biology). View Reference
550503 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.