-
Your selected country is
Canada
- Change country/language
-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
- Immunoassay Reagents
-
Single-Cell Multiomics Reagents
- BD® OMICS-Guard Sample Preservation Buffer
- BD® AbSeq Assay
- BD® OMICS-One Immune Profiler Protein Panel
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Accessory Kits
-
Functional Assays
-
Microscopy and Imaging Reagents
-
Cell Preparation and Separation Reagents
-
Dehydrated Culture Media
-
- BD® OMICS-Guard Sample Preservation Buffer
- BD® AbSeq Assay
- BD® OMICS-One Immune Profiler Protein Panel
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Accessory Kits
- Canada (English)
- Change country/language
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Fluorochrome Faceoff
Watch your favorite fluorochromes take on the competition!
Game 4: Intracellular Staining
-
When you assess intracellular markers in your assay, fluorochrome choice can have a significant impact on your results. Not all fluorochromes work well for intracellular staining, as some fluorochromes are impacted by buffers used to fix and permeabilize the cells.
In this Fluorochrome Faceoff, we pair fluorochromes that are excited off the blue laser and emit around 610 nm. We selected several different intracellular markers to evaluate and stained them with three different fluorochromes. As you can see, each dye provides good population resolution and the expected staining pattern. Please note that there are clone differences between the dyes.
Winner’s Circle
Because all three of the dyes tested perform well for intracellular staining, we have classified them all as winners! Please note that as of July 26, 2024, StarBright™ and NovaFluor™ Dye-conjugated antibodies are currently only available against cell surface markers and are not currently conjugated to intracellular markers. They were, therefore, not included in this Fluorochrome Faceoff.
PRO TIP: In addition to fluorochrome sensitivity, different clones are also differentially impacted by treatments with different permeabilization protocols and therefore it is important to make sure you are using an antibody clone that can withstand these protocols.
Try the Winning Fluorochrome
Request a sample of RB613
*Required fields
Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.
BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.