BD Transduction Laboratories™ Purified Mouse Anti-MnSOD
Clone 19/MnSOD (RUO)
Western blot analysis of MnSOD on a mouse kidney lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti- MnSOD antibody.
Western blot analysis of MnSOD on a mouse kidney lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti- MnSOD antibody.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese (Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2 and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to toxic stimuli is thought to be critical for the survival of a variety of cell types.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Ambrosone CB, Freudenheim JL, Thompson PA. Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res. 1999; 59(3):602-606. (Biology). View Reference
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Gilks CB, Price K, Wright JL, Churg A. Antioxidant gene expression in rat lung after exposure to cigarette smoke. Am J Pathol. 1998; 152(1):269-278. (Biology). View Reference
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Gonzalez-Zulueta M, Ensz LM, Mukhina G. Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity. J Neurosci. 1998; 18(6):2040-2055. (Biology). View Reference
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Kim HP, Roe JH, Chock PB, Yim MB. Transcriptional activation of the human manganese superoxide dismutase gene mediated by tetradecanoylphorbol acetate. J Biol Chem. 1999; 274(52):37455-37460. (Biology). View Reference
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Rohrdanz E, Kahl R. Alterations of antioxidant enzyme expression in response to hydrogen peroxide. Free Radic Biol Med. 1998; 24(1):27-38. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.