BD Pharmingen™ Purified Mouse anti-Human Rab27
Clone 20/RAB27 (RUO)
Western blot analysis of Rab27 in human chronic myelogenous leukemia. Lysate from the K-562 cell line (Cat. No. 611550) was probed with Purified Mouse anti-Human Rab27 (Cat. No. 558532) at concentrations of 1.00, 0.50, and 0.25 µg/ml (Lanes 1, 2, and 3, respectively). Visualization was carried out with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Rab27 is identified as a band of 25 kDa.
Western blot analysis of Rab27 in human chronic myelogenous leukemia. Lysate from the K-562 cell line (Cat. No. 611550) was probed with Purified Mouse anti-Human Rab27 (Cat. No. 558532) at concentrations of 1.00, 0.50, and 0.25 µg/ml (Lanes 1, 2, and 3, respectively). Visualization was carried out with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Rab27 is identified as a band of 25 kDa.
Preparation And Storage
Product Notices
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Members of the Rab family of GTPases are non-transforming, membrane-bound, geranylgeranylated members of the Ras superfamily that are involved in the fusion and trafficking of cellular vesicles. Rab27A is expressed in many human tissues and it regulates the secretion of several types of cellular vesicles, such as melanosomes, insulin granules of pancreatic beta cells, and lytic granules of cytotoxic T lymphocytes. Rab27A is able to regulate such diverse processes by interacting with organelle-specific effector proteins, including melanophilin, granuphilin, and Munc13-4, respectively. Defects in Rab27A function are associated with the human diseases choroideremia and Griscelli syndrome 2 and the ashen mutant mouse. Rab27B is not as widely expressed, yet it is reported to have similar functional capacities as Rab27A.
The 20.1 monoclonal antibody recognizes human Rab27A; cross-reactivity with Rab27B is unknown.
Development References (4)
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Fukuda M. Versatile role of Rab17 in membrane trafficking: Focus on the Rab27 effector families. J Biochem (Tokyo). 2005; 137:9-16. (Biology).
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Hong W. Cytotoxic T lymphocyte exocytosis: bring on the SNAREs!. Trends Cell Biol. 2005; 15(12):644-50. (Biology). View Reference
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Izumi T, Gomi H, Kasai K, Mizutani S, Torii S. The roles of Rab27 and its effectors in the regulated secretory pathways. Cell Struct Funct. 2003; 28:465-474. (Biology). View Reference
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McKusick VA, Hartz PA, Stone DL, Antonarakis SE, O'Neill MJF. Ras-associated protein Rab27A; Rab27A. Available: http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=603868 2006, May 23.
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