BD Transduction Laboratories™ Purified Mouse Anti-HERC2
Clone 17/HERC2 (RUO)
Western blot analysis of HERC2 on mouse cerebellum. Lane 1: 250, lane 2:5000, land 3: 1:1000 dilution of HERC2.
Western blot analysis of HERC2 on mouse cerebellum. Lane 1: 250, lane 2:5000, land 3: 1:1000 dilution of HERC2.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Prader-Willi and Angelman syndromes are genetic disorders that result from recombination between chromsome-specific low-copy repeats (duplicons). ERC2(rjs) is the transcript found in the duplicon for these disorders, and recessive mutation of HERC2 leads to a developmental syndrome in mice, referred to as runty jerky sterile (rjs) and juvenile development and fertility 2 (jdf2). HERC2 is homologous to the HERC1 and HERC3 HECT-domain containing proteins. The sequence of HERC2 includes three RCC1-like domains (RLD), a C-terminal ECT domain, and a ZZ-type zinc finger. The RCC1 repeats are similar to those found in cytochrome b5 and the HECT domain is found in E6-AP ubiquitin ligase. HERC2 may function as both a guanine nucleotide exchange factor and E3 biquitin ligase based on its conserved motifs and observation from mouse mutation studies. HERC2 mRNA is expressed highest in brain and testes, but is also found in heart, lung, liver, skeletal muscle, and kidney. Thus, HERC2 may function in protein trafficking and degradation pathways in various tissues.
Development References (2)
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Ji Y, Rebert NA, Joslin JM, Higgins MJ, Schultz RA, Nicholls RD. Structure of the highly conserved HERC2 gene and of multiple partially duplicated paralogs in human. Genome Res. 2000; 10(3):319-329. (Clone-specific: Western blot). View Reference
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Lehman AL, Nakatsu Y, Ching A. A very large protein with diverse functional motifs is deficient in rjs (runty, jerky, sterile) mice. Proc Natl Acad Sci U S A. 1998; 95(16):9436-9441. (Biology). View Reference
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