BD Pharmingen™ Purified Mouse Anti-ERK1
Clone G262-118 (RUO)
Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.
Western blot analysis of ERK1. RSVtransformed mouse 3T3 fibroblasts were probed with anti-ERK1 (clone G262-118) at concentrations of 0.2 (lane 1), 0.04 (lane 2), and 0.008 µg/ml (lane 3). ERK1 is detected at ~44 kDa.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
A serine/threonine specific protein kinase, called MAP kinase or more recently ERK1 (extracellular signal regulated kinase) is activated in cells following stimulation with EGF (epidermal growth factor), the PDGF (platelet-derived factor) or insulin. ERK1 has been assayed in vitro for phosphotransferase activity using the microtubule-associated protein (MAP) or myelin basic protein. The mechanism by which MAP kinase becomes activated in growth factor treated cells is thought to involve tyrosine and threonine phosphorylation of the kinase itself. The EGF, PDGF and insulin receptors are known to be tyrosine specific protein kinases and studies have shown that MAP kinase is identical to the p42 protein that earlier studies identified as one of the major tyrosine phosphorylated proteins in transformed or growth factor treated cells. While several isoforms of the p42 protein are known, they are thought to be structurally similar. The G262-118 antibody recognizes human and mouse ERK1. It does not cross-react with ERK2. A synthetic peptide from the C-terminal region of ERK1 was used as immunogen.
Development References (2)
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Campos-Gonzalez R, Glenney JR Jr. Temperature-dependent tyrosine phosphorylation of microtubule-associated protein kinase in epidermal growth factor-stimulated human fibroblasts. Cell Regul. 1993; 2(8):663-673. (Biology). View Reference
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Rossomando AJ, Payne DM, Weber MJ, Sturgill TW. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase. Proc Natl Acad Sci U S A. 1989; 86(18):6940-6943. (Biology). View Reference
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