BD Pharmingen™ Purified Mouse Anti- Bcl-x
Clone 2H12 (RUO)
Western blot analysis of Bcl-x expression on Jurkat cell lysate. Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with Purified Mouse Anti- Bcl-x antibody (Cat. No. 551022/551020) at concentrations of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3), and visualized with HRP Goat Anti-Mouse Ig secondary antibody (Cat. No. 554002).
Western blot analysis of Bcl-x expression on Jurkat cell lysate. Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) was probed with Purified Mouse Anti- Bcl-x antibody (Cat. No. 551022/551020) at concentrations of 2.0 µg/mL (lane 1), 1.0 µg/mL (lane 2), and 0.5 µg/mL (lane 3), and visualized with HRP Goat Anti-Mouse Ig secondary antibody (Cat. No. 554002).
Description
Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death.
The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.
Preparation And Storage
Recommended Assay Procedures
In addition to Jurkat cell lysates, MOLT-4 (Human T-lymphoblasts; ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia; ATCC CCL-243), Hut-78 (Human T lymphoma; ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| N/A | 50.0 | N/A | N/A |
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.