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      HRP Protein A
      Product Details
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      BD Transduction Laboratories™
      Western blot (Routinely Tested)
      1.0 mg/ml
      AB_2870028
      1 mg/ml in 50% glycerol, 20mM sodium phosphate pH 7.5, 150 mM NaCl.
      RUO


      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Horseradish_Peroxidase.shtml

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      610438 Rev. 1
      Antibody Details
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      Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.  

      Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested.

      610438 Rev. 1
      Format Details
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      HRP
      Horseradish Peroxidase (HRP) is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide resulting in a visible colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with HRP and used in applications such as i) ELISA that uses a plate reader to measure absorbance of the colored product, or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
      HRP
      610438 Rev.1
      Citations & References
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      View product citations for antibody "610438" on CiteAb
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      No Citations Are Available for this Product

      610438 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.