BD Transduction Laboratories™ HRP Mouse Anti-Phosphotyrosine
Clone PY20 (RUO)
Western blot analysis of phosphotyrosine on A431 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-phosphotyrosine, PY20.
Western blot analysis of phosphotyrosine on A431 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-phosphotyrosine, PY20.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.
Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.
Development References (5)
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Alderton F, Rakhit S, Kong KC, et al. Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells. J Biol Chem. 2001; 276(30):28578-28585. (Clone-specific: Western blot). View Reference
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Borges E, Jan Y, Ruoslahti E. Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain. J Biol Chem. 2000; 275(51):39867-39873. (Clone-specific: Western blot). View Reference
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Goitsuka R, Tatsuno A, Ishiai M, Kurosaki T, Kitamura D. MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT. J Biol Chem. 2001; 276(38):36043-36050. (Clone-specific: Western blot). View Reference
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Hemmeryckx B, Reichert A, Watanabe M, et al. BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl. Oncogene. 2002; 21(20):3225-3231. (Clone-specific: Western blot). View Reference
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Smith AJ, Surviladze Z, Gaudet EA, Backer JM, Mitchell CA, Wilson BS. p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production. J Biol Chem. 2001; 276(20):17213-17220. (Clone-specific: Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.