Preparation And Storage
Recommended Assay Procedures
Applications include immunoprecipitation and western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB 152) are suggested as a positive control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked α-β heterodimer. A minor fraction express Ti as a γ-δ heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, γ (gamma), δ (delta), ε (epsilon), ζ (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer), and 21 kD respectively. 1D4 recognizes human but not mouse CD3ζ. It is most efficient at recognizing the phosphorylated form of CD3ζ (21 kD) but will also bind the 16 kD unphosphorylated monomer as well as higher molecular weight dimers. A synthetic peptide corresponding to amino acids 153 to 164 of human CD3ζ was used as immunogen.
Development References (3)
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Hall CG, Sancho J, Terhorst C. Reconstitution of T cell receptor zeta-mediated calcium mobilization in nonlymphoid cells. Science. 1993; 261(5123):915-918. (Immunogen: Immunoprecipitation, Western blot). View Reference
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Hussey RE, Clayton LK, Diener A, et al. Overexpression of CD3 eta during thymic development does not alter the negative selection process. J Immunol. 1993; 150(4):1183-1194. (Biology). View Reference
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Sancho J, Franco R, Chatila T, Hall C, Terhorst C. The T cell receptor associated CD3-epsilon protein is phosphorylated upon T cell activation in the two tyrosine residues of a conserved signal transduction motif. Eur J Immunol. 1993; 23(7):1636-1642. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.