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      Purified Rat Anti-Mouse TER-119/Erythroid Cells
      75281E_550565_image1.png

      Immunohistochemical staining of mouse erythroid cells. Formalin-fixed paraffin sections of normal mouse kidney were reacted with the anti-TER-119 antibody. Cells of the erythroid origin can be identified by the brown labeling of their cell membranes. Amplification 20X.

      75281E_550565_image1.png Blue-Cap-White-Label.jpg

      Immunohistochemical staining of mouse erythroid cells. Formalin-fixed paraffin sections of normal mouse kidney were reacted with the anti-TER-119 antibody. Cells of the erythroid origin can be identified by the brown labeling of their cell membranes. Amplification 20X.

      Product Details
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      BD Pharmingen™
      Lymphocyte antigen 76; Ly76; Ly-76; TER-119; Ter119
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2b, κ
      Mouse Fetal Liver
      Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
      31.25 µg/ml
      AB_393756
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      Immunohistochemistry: The TER-119 antibody specific for mouse erythroid cells is recommended to test for immunohistochemical staining of formalin-fixed paraffin and acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and kidney. The antibody stains cells of the erythroid origin. The isotype control recommended for use with this antibody is purified rat IgG2b (Cat. No. 559478). For optimal indirect immunohistochemical staining, the TER-119 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with, biotin conjugated anti-rat IgG2b (Cat. No. 550327) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550565 Rev. 2
      Antibody Details
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      TER-119

      The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the "lineage cocktail" used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages.

      550565 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550565 Rev.2
      Citations & References
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      View product citations for antibody "550565" on CiteAb

      Development References (3)

      1. Ikuta K, Kina T, MacNeil I, et al. A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells. Cell. 1990; 62(5):863-874. (Biology). View Reference
      2. Kina T, Ikuta K, Takayama E, et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol. 2000; 109(2):280-287. (Immunogen). View Reference
      3. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.
      550565 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.