BD Pharmingen™ Purified Mouse Anti-Tie2
Clone 33 (also known as MoAb33; Ab33) (RUO)
Western blot analysis for Tie2. Human endothelial cell lysates were probed with the mouse anti-Tie2 antibody at concentrations of 4 µg/mL (lane 1), 2 µg/mL (lane 2), and 1 µg/mL (lane 3). Due to glycosylation, Tie2 may be observable migrating in a range between ~130-165 kDa.
Western blot analysis for Tie2. Human endothelial cell lysates were probed with the mouse anti-Tie2 antibody at concentrations of 4 µg/mL (lane 1), 2 µg/mL (lane 2), and 1 µg/mL (lane 3). Due to glycosylation, Tie2 may be observable migrating in a range between ~130-165 kDa.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The 33 monoclonal antibody specifically recognizes Tunica interna endothelial cell kinase-2 (Tie2) which is also known as CD202b or Angiopoietin-1 receptor. Tie2 (CD202b) is a single-pass type I membrane protein that is encoded by TEK (TEK receptor tyrosine kinase) which belongs to the receptor tyrosine kinase (RTK) family. The extracellular region of Tie2 (CD202b) contains an N-terminal IgC2-like domain followed by three EGF-like domains, an additional IgC2-like domain and three fibronectin type III domains. The intracellular region contains a protein kinase domain. TIE receptors, designated Tie1 and Tie2 (Tek) have a tissue distribution which is limited primarily to cells of endothelial origin. Binding of angiopoietin 1 to Tie2 (CD202b) leads to downstream receptor-mediated signaling that is involved in angiopoiesis and survival of endothelial cells. Tie2 has been shown to be required for the normal development of vascular structures during embryogenesis. It has also been demonstrated to play a role in normal and pathological angiogenesis in adults. Mutations in the TEK gene cause multiple cutaneous and mucosal venous malformations (VMCM). Tie2 has a predicted molecular weight of 126 kD, but may be observable at ~130-165 kDa. This antibody has been reported to recognize human and mouse Tie2 but not Tie1.
Development References (4)
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Dumont DJ, Gradwohl G, Fong GH, et al. Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo. Genes Dev. 1994; 8(16):1897-1909. (Biology). View Reference
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Partanen J, Dumont DJ. Functions of Tie1 and Tie2 receptor tyrosine kinases in vascular development. Curr Top Microbiol Immunol. 1999; 237:159-172. (Biology). View Reference
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Peters KG, Coogan A, Berry D, et al. Expression of Tie2/Tek in breast tumour vasculature provides a new marker for evaluation of tumour angiogenesis. Br J Cancer. 1998; 77(1):51-56. (Immunogen: Immunohistochemistry, Western blot). View Reference
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Wong AL, Haroon ZA, Werner S, Dewhirst MW, Greenberg CS, Peters KG. Tie2 expression and phosphorylation in angiogenic and quiescent adult tissues. Circ Res. 1997; 81(4):567-574. (Biology: Immunohistochemistry, Western blot). View Reference
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