BD Transduction Laboratories™ Purified Mouse Anti-Rab11
Clone 47/Rab11 (RUO)
Western blot analysis of Rab11 on MDCK lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-MDCK.
Western blot analysis of Rab11 on MDCK lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-MDCK.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The Rab proteins are small GTP-binding molecules that are localized to specific intracellular vesicles and organelles. It has been proposed that Rab proteins cycle between GTP- and GDP-bound forms and that this is related to their function as regulators of vesicular traffic. The Rab11 gene encodes a 24 kDa protein of 214 amino acids that has been detected in liver, brain, testis, spleen, and heart. Rab11 protein was isolated from the golgi-microsomal fraction of rat liver and has been detected in the Trans-golgi Network, secretory vesicles, and the pericentriolar recycling endosomes. The distribution of Rab11 indicates that this small protein is involved in regulating traffic at the Golgi complex.
Development References (5)
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Choudhury A, Dominguez M, Puri V, et al. Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cells. J Clin Invest. 2002; 109(12):1541-1550. (Clone-specific: Western blot). View Reference
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Lai F, Stubbs L, Artzt K. Molecular analysis of mouse Rab11b: a new type of mammalian YPT/Rab protein. Genomics. 1994; 22(3):610-616. (Biology). View Reference
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Sakurada K, Uchida K, Yamaguchi K, et al. Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver. Biochem Biophys Res Commun. 1991; 177(3):1224-1232. (Biology). View Reference
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Steiner P, Sarria JC, Glauser L, Magnin S, Catsicas S, Hirling H. Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. J Cell Biol. 2002; 157(7):1197-1209. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Woods AJ, Roberts MS, Choudhary J, et al. Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells. J Biol Chem. 2002; 277(8):6428-6437. (Clone-specific: Western blot). View Reference
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