BD Pharmingen™ Purified Mouse Anti-MyoD
Clone 5.8A (RUO)
Western blot analysis of MyoD expression on Rh30 Rhabdomyosarcoma cells. Rh30 cell lysate was stained with Purified Mouse Anti-MyoD (Cat. No. 554130), followed by HRP Goat Anti-Mouse Ig (Cat. No. 554130) for 1 hour at room temperature. MyoD is identified as a 45-48 kDa band.
Western blot analysis of MyoD expression on Rh30 Rhabdomyosarcoma cells. Rh30 cell lysate was stained with Purified Mouse Anti-MyoD (Cat. No. 554130), followed by HRP Goat Anti-Mouse Ig (Cat. No. 554130) for 1 hour at room temperature. MyoD is identified as a 45-48 kDa band.
Preparation And Storage
Recommended Assay Procedures
Applications include Western blot analysis (1-2 µg/ml) and immunohistochemical staining of paraformaldehyde-fixed, frozen tissue sections and myogenic cell lines (titrate between 0.5-5 µg/ml). RD Rhabdomysarcoma cells (ATCC CCL-136) and normal myoblasts are suggested as a positive control for these applications. Cell lines which are suggested as negative controls include: Ewing's sarcoma cells RD-ES (ATCC HBT-166), neuroblastoma cells SK-N-SH (ATCC HTB-11), and Burkitts lymphoma cells EB2 (ATCC HTB-61).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development; for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade.
The epitope for MoAb 5.8A has been mapped to amino acids 180-189 of mouse MyoD.
Development References (5)
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Davis RL, Weintraub H, Lassar AB. Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 1987; 51(6):987-1000. (Biology). View Reference
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Dias P, Dilling M, Houghton P. The molecular basis of skeletal muscle differentiation.. Semin Diagn Pathol. 1994; 11(1):3-14. (Biology). View Reference
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Dias P, Parham DM, Shapiro DN, Tapscott SJ, Houghton PJ. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Res. 1992; 52(23):6431-6439. (Clone-specific). View Reference
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Gredinger E, Gerber AN, Tamir Y, Tapscott SJ, Bengal E. Mitogen-activated protein kinase pathway is involved in the differentiation of muscle cells. J Biol Chem. 1998; 273(17):10436-10444. (Biology). View Reference
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Rawls A, Valdez MR, Zhang W, Richardson J, Klein WH, Olson EN. Overlapping functions of the myogenic bHLH genes MRF4 and MyoD revealed in double mutant mice. Development. 1998; 125(13):2349-2358. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.