BD Transduction Laboratories™ Purified Mouse Anti-N-Cadherin
Clone 32/N-Cadherin (RUO)
Western blot analysis of N-Cadherin on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000, lane 4: 1:2000 dilution of anti-N-Cadherin.
Immunofluorescent staining of 293 cells with anti-N-Cadherin.
Western blot analysis of N-Cadherin on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000, lane 4: 1:2000 dilution of anti-N-Cadherin.
Immunofluorescent staining of 293 cells with anti-N-Cadherin.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Cadherins are a family of Ca2+-dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ). Members of the cadherin family include P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Development References (5)
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Bhowmick NA, Ghiassi M, Bakin A. Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell. 2001; 12(1):27-36. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Cowin P. Unraveling the cytoplasmic interactions of the cadherin superfamily. Proc Natl Acad Sci U S A. 1994; 91(23):10759-10761. (Biology). View Reference
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Izawa I, Nishizawa M, Ohtakara K, Inagaki M. Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses. J Biol Chem. 2002; 277(7):5345-5350. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Miyatani S, Shimamura K, Hatta M. Neural cadherin: role in selective cell-cell adhesion. Science. 1989; 245(4918):631-635. (Biology). View Reference
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Nurnberger J, Bacallao RL, Phillips CL. Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells. Mol Biol Cell. 2002; 13(9):3096-3106. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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