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      Purified Mouse Anti-JBP1/Skb1Hs
      Product Details
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
      Mouse IgG1
      Human Skb1Hs aa. 423-624
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
      72 kDa
      250 µg/ml
      AB_398997
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      611539 Rev. 1
      Antibody Details
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      32/JBP1

      p21[Cdc42/Rac]-activated kinases (PAKs) bind and are activated by the small GTPases, Cdc42 and Rac. In yeast, the interaction of the PAK, Shk1, with Skb1 (Shk1 kinase binding protein 1) is important in the regulation of cell morphology and mitosis. Skb1Hs, the human homolog of Skb1, restores growth rates in yeast cells deficient for Skb1. Skb1Hs has also been identified as a 72 kDa pICln-binding protein (IBP72). pICln is thought to be a cytosolic regulator of a swelling-induced chloride channel and interactions with Skb1Hs suggest a link with PAKs, which are involved in cytoskeletal rearrangement. An additional line of study has identified Skb1Hs as a Jak binding protein (JBP1) via its interactions with Jak1-3 and Tyk2. This study also identified Skb1Hs as a methyltransferase that interacts with multiple proteins which possibly serve as physiological targets. Skb1Hs is expressed in a wide range of tissues and is thought to form homodimeric or multimeric complexes. Thus, Skb1Hs (IBP72, JBP1) is a multifunctional protein that may participate in such diverse processes as transcriptional and translational regulation, cytoskeletal rearrangement, and any of a number of signaling pathways.

      611539 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      611539 Rev.1
      Citations & References
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      View product citations for antibody "611539" on CiteAb

      Development References (3)

      1. Gilbreth M, Yang P, Bartholomeusz G, et al. Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. Proc Natl Acad Sci U S A. 1998; 95(25):14781-14786. (Biology). View Reference
      2. Krapivinsky G, Pu W, Wickman K, Krapivinsky L, Clapham DE. pICln binds to a mammalian homolog of a yeast protein involved in regulation of cell morphology. J Biol Chem. 1998; 273(18):10811-10814. (Biology). View Reference
      3. Pollack BP, Kotenko SV, He W, Izotova LS, Barnoski BL, Pestka S. The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity. J Biol Chem. 1999; 274(44):31531-31542. (Biology). View Reference
      611539 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.