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BD Transduction Laboratories™ Purified Mouse Anti-hSlu7
Clone 35/hSlu7 (RUO)
Western blot analysis of hSlu7 on K-562 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of hSlu7.
MDCK
Western blot analysis of hSlu7 on K-562 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of hSlu7.
MDCK
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Reactivity Confirmed in Development), Chicken (Lack of Reactivity Confirmed in Development)
Mouse IgG2b
Human hSlu7 aa. 457-570
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80 kDa
250 µg/ml
AB_399887
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612604 Rev. 1
Antibody Details
35/hSlu7
Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3' splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), Prp18p (hPrp18) and Slu7 (hSlu7). hSlu7 contains a zinc knuckle motif similar to the yeast Slu7. This domain is present in retroviral nucleocapsid proteins and in several splicing factors. hSlu7 associates with the spliceosome late in the splicing pathway during recognition of the 3' splice site. During step II of 3' splicing, hSlu7 tightly binds to exon 1 in the spliceosome and helps specify attack on the correct adenine-guanine dinucleotide, located 18 to 40 nucleotides downstream of the branch site.
612604 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612604 Rev.1
Citations & References
View product citations for antibody "612604" on CiteAb
Development References (2)
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Chua K, Reed R. Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing. Genes Dev. 1999; 13(7):841-850. (Biology). View Reference
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Chua K, Reed R. The RNA splicing factor hSlu7 is required for correct 3' splice-site choice. Nature. 1999; 402(6758):207-210. (Biology). View Reference
612604 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.