BD Transduction Laboratories™ Purified Mouse Anti-HAX-1
Clone 52/HAX-1 (RUO)
Western blot analysis of HAX-1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HAX-1 antibody.
Immunofluorescent staining of HeLa cells with anti-HAX-1 antibody.
Western blot analysis of HAX-1 on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-HAX-1 antibody.
Immunofluorescent staining of HeLa cells with anti-HAX-1 antibody.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Cell-cell interactions in the immune system, mediated by membrane-bound antigen receptors, trigger a cascade of signaling mechanisms collectively known as the T-cell response. Upon antigen binding, cellular tyrosine kinases are activated resulting in the phosphorylation of HS1 or Lck-binding protein 1 (LckBP1), which is involved in thymic negative selection. HS1 is one of the earliest intracellular proteins to be tyrosine phosphorylated in response to antigen-receptor cross-linking in T cells. HAX-1 (HS1-associated protein X-1) is a 35 kDa protein found associated with HS1 by co-immunoprecipitation assays and the two-hybrid system. HAX-1 is ubiquitously expressed and localized in the mitochondria, endoplasmic reticulum, and the nuclear envelope. The HS1-HAX-1 complex may be critical for signal transduction pathways initiated by antigen presentation to T cells.
Development References (1)
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Suzuki Y, Demoliere C, Kitamura D, Takeshita H, Deuschle U, Watanabe T. HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases. J Immunol. 1997; 158(6):2736-2744. (Biology). View Reference
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