BD Transduction Laboratories™ Purified Mouse Anti-Caveolin 2
Clone 65/Caveolin 2 (RUO)
Western blot analysis of Caveolin 2 on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Caveolin 2 antibody.
Immunofluorescence staining of FHs cells (Normal human fetal lung fibroblasts; ATCC HTB-157).
Western blot analysis of Caveolin 2 on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Caveolin 2 antibody.
Immunofluorescence staining of FHs cells (Normal human fetal lung fibroblasts; ATCC HTB-157).
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Identified as a tyrosine phosphorylated protein in Rous sarcoma virus- transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN) and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin is part of a complex containing glycosylphosphatidylinositol (GPI)-linked molecules and cytoplasmic signaling proteins. Caveolin is a transmembrane adaptor molecule that can simultaneously recognize GPI-linked proteins and interact with downstream cytoplasmic signaling molecules, such as c-yes, Annexin II, and hetero-trimeric G proteins. Although caveolin 2 is similar to caveolin 1 in distribution and tissue expression, caveolin 2 is most abundant in adipose tissue and its expression is up-regulated upon differentiation. This antibody has been reported to recognize an epitope located within region 79-88 of caveolin 2.
Development References (6)
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Das K, Lewis R, Scherer P, Lisanti M. The membrane-spanning domains of caveolins-1 and -2 mediate the formation of caveolin hetero-oligomers. J Biol Chem. 1999; 274(26):18721-18728. (Immunogen).
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Kiss AL, Turi A, Mullner N, Timar J. Caveolin isoforms in resident and elicited rat peritoneal macrophages. Eur J Cell Biol. 2000; 79(5):343-349. (Biology: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Scherer PE, Okamoto T, Chun M, Nishimoto I, Lodish HF, Lisanti MP. Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc Natl Acad Sci U S A. 1996; 93(1):131-135. (Biology). View Reference
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Tauchi-Sato K, Ozeki S, Houjou T, Taguchi R, Fujimoto T. The surface of lipid droplets is a phospholipid monolayer with a unique Fatty Acid composition. J Biol Chem. 2002; 277(46):44507-44512. (Biology: Electron microscopy, Western blot). View Reference
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Woodman SE, Park DS, Cohen AW, et al. Caveolin-3 knock-out mice develop a progressive cardiomyopathy and show hyperactivation of the p42/44 MAPK cascade. J Biol Chem. 2002; 277(41):38988-38997. (Biology: Immunofluorescence, Western blot). View Reference
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Zschocke J, Manthey D, Bayatti N, van der Burg B, Goodenough S, Behl C. Estrogen receptor alpha-mediated silencing of caveolin gene expression in neuronal cells. J Biol Chem. 2002; 277(41):38772-38780. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
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