BD Transduction Laboratories™ Purified Mouse Anti-Bcl-x
Clone 44/Bcl-x (RUO)
Western blot analysis of Bcl-x on human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Bcl-x.
Immunofluorescent staining of Jurkat cells.
Western blot analysis of Bcl-x on human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Bcl-x.
Immunofluorescent staining of Jurkat cells.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Bcl-x is related to the Bcl-2 protein and can function independently of Bcl-2 in regulating apoptosis (programmed cell death). The bcl-x transcript is expressed in a number of tissues, with the highest levels in the lymphoid and central nervous systems. Two distinct cDNA species, bcl-xL and bcl-xS, have been observed and appear to arise from alternate 5' splice sites located within the first coding exon of the bcl-x gene. Bcl-xL is composed of 233 amino acids and is similar in size and structure to Bcl-2. The Bcl-xS polypeptide shows a deletion of 63 amino acids which consist of the region of Bcl-xL with the highest degree of amino acid identity to Bcl-2. Like Bcl-2, Bcl-xL inhibits cell death upon growth factor withdrawal when transfected into an IL-3-dependent cell line. However, Bcl-xS inhibits Bcl-2 mediated cell survival.
Development References (5)
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Anderson JS, Teutsch M, Dong Z, Wortis HH. An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis. Proc Natl Acad Sci U S A. 1996; 93(26):10966-10971. (Biology). View Reference
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Devarajan P, De Leon M, Talasazan F, Schoenfeld AR, Davidowitz EJ, Burk RD. The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways. J Biol Chem. 2001; 276(44):40599-40605. (Clone-specific: Western blot). View Reference
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Kishimoto H, Sprent J. Strong TCR ligation without costimulation causes rapid onset of Fas-dependent apoptosis of naive murine CD4+ T cells. J Immunol. 1999; 163(4):1817-1826. (Clone-specific: Flow cytometry). View Reference
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Oetzel C, Jonuleit T, Gotz A, et al. The tyrosine kinase inhibitor CGP 57148 (ST1 571) induces apoptosis in BCR-ABL-positive cells by down-regulating BCL-X. Clin Cancer Res. 2000; 6(5):1958-1968. (Clone-specific: Western blot). View Reference
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Soini Y, Kinnula V, Kaarteenaho-Wiik R, Kurttila E, Linnainmaa K, Paakko P. Apoptosis and expression of apoptosis regulating proteins bcl-2, mcl-1, bcl-X, and bax in malignant mesothelioma. Clin Cancer Res. 1999; 5(11):3508-3515. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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