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      Purified NA/LE Rat Anti-Mouse IL-2
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat IgG2a
      T cell clone supernatant
      ELISA (Routinely Tested), Neutralization (Tested During Development), Western blot (Reported)
      1.0 mg/ml
      AB_395355
      No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      Western Blot:  Purified NA/LE Rat Anti-Mouse IL-2 has been reported to be useful for Western blotting.  A concentration of 1-5 µg/mL (in conjunction with an AKP-conjugated goat anti-rat Ig secondary antibody) has been reported to enable detection of  ≤ 100 ng/lane of recombinant mouse IL-2 (Cat. No. 550069) under reducing conditions.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554375 Rev. 6
      Antibody Details
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      S4B6

      The S4B6 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the S4B6 hyridoma was a T cell clone supernatant. This is a neutralizing antibody.

      554375 Rev. 6
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is =0.01 EU/µg (=0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554375 Rev.6
      Citations & References
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      View product citations for antibody "554375" on CiteAb

      Development References (5)

      1. Cherwinski HM, Schumacher JH, Brown KD, Mosmann TR. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987; 166(5):1229-1244. (Clone-specific: Neutralization). View Reference
      2. Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Methodology: Bioassay). View Reference
      3. Kubo M, Cinader B. Polymorphism of age-related changes in interleukin (IL) production: differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4. Eur J Immunol. 1990; 20(6):1289-1296. (Biology). View Reference
      4. Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986; 136(7):2348-2357. (Clone-specific: Neutralization). View Reference
      5. Svetic A, Jian YC, Lu P, Finkelman FD, Gause WC. Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells. Int Immunol. 1993; 5(8):877-883. (Clone-specific: Neutralization). View Reference
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      554375 Rev. 6

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.