BD Pharmingen™ Purified Mouse Anti-Itk
Clone 2F12 (RUO)
Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, Cat. No. 550503) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Itk is identified as a band at ~72 kDa.
Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.
Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clone 2F12, Cat. No. 550503) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Itk is identified as a band at ~72 kDa.
Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were first immunoprecipitated (2 µg/1 x 10^6 cells) with anti-Itk (clone 2F12, Cat. No. 550503) and then the western blot was probed with the same antibody at a concentration of 0.5 µg/ml. Itk is identified as a band of ~72 kDa.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
Development References (3)
-
Heyeck SD, Wilcox HM, Bunnell SC, Berg LJ.. Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity.. J Biol Chem. 272(25401)(Immunogen: Immunoprecipitation, In vitro kinase assay, Western blot).
-
Liu KQ, Bunnell SC, Gurniak CB, Berg LJ. T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells. J Exp Med. 1998; 187(10):1721-1727. (Clone-specific: Western blot). View Reference
-
Siliciano JD, Morrow TA, Desiderio SV. itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2. Proc Natl Acad Sci U S A. 1992; 89(23):11194-11198. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.