BD Pharmingen™ Purified Mouse Anti-Human IL-8
Clone G265-5 (RUO)
Human IL-8 sandwich ELISA standard curves. The curves were generated by sandwich ELISAs using Purified Mouse Anti-Human IL-8 (Cat. No. 554716) as capture antibody, doubling dilutions of recombinant IL-8 standards, and Biotin Mouse Anti-Human IL-8 (Cat. No. 554718) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA. Note that several distinct N-termini exist in IL-8; standard curves were generated with recombinant 72 amino acid (open square curve) or 77 amino acid (filled circle curve) IL-8 standards.
Human IL-8 sandwich ELISA standard curves. The curves were generated by sandwich ELISAs using Purified Mouse Anti-Human IL-8 (Cat. No. 554716) as capture antibody, doubling dilutions of recombinant IL-8 standards, and Biotin Mouse Anti-Human IL-8 (Cat. No. 554718) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA. Note that several distinct N-termini exist in IL-8; standard curves were generated with recombinant 72 amino acid (open square curve) or 77 amino acid (filled circle curve) IL-8 standards.
Preparation And Storage
Recommended Assay Procedures
ELISA Capture: For a sandwich ELISA to measure human IL-8 protein levels, the Purified Mouse Anti-Human IL-8 (Cat. No. 554716) can be paired with the Biotin Mouse Anti-Human IL-8 (Cat. No. 554718), with Recombinant Human IL-8 (Cat. No. 554609) as the standard. Purified Mouse Anti-Human IL-8 should be titrated from 1 - 4 µg/ml to determine the optimal concentration for ELISA capture. To obtain standard curves, doubling dilutions of human IL-8 protein ranging from ~1,000 to 1 pg/ml are recommended for inclusion in each ELISA plate. It is recommended that the investigator should prepare dilutions of the sample to ensure that the level of IL-8 falls within this range. For specific methodology, please visit the protocols under "ELISA and ELISPOT" at our website, http://www.bdbiosciences.com/us/s/resources.
This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IL-8 in serum or plasma our mouse IL-8 BD OptEIA™ Set (Cat. No. 555244) or BD OptEIA Kit (Cat. No. 550999) are specially formulated and recommended.
This ELISA pair shows no cross-reactivity with any other cytokines (e.g., human IL-1α, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFN-γ, TNF, LT-α, MCP-1, MCP-2, MIP-1α, MIP-1ß, SCF, lymphotactin, VEGF, G-CSF, NT-3, PDGF, CD23, IP-10, GRO, NAP-2, PF4).
Western Blot: The clone G265-5 has been reported to be useful for Western Blotting. Please note that this application is not routinely tested at BD Biosciences.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was recombinant human IL-8.
Development References (1)
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Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Clone-specific). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.