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      HRP Rat Anti-Mouse Ig, κ Light Chain
      Product Details
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      BD Pharmingen™
      Ig kappa chain C region; IGKC; Igk-C; Ig, κ
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
      Mouse IgG2b κ secreted by MPC-11 plasmacytoma
      ELISA (Routinely Tested)
      AB_397315
      Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
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      Recommended Assay Procedures

      Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000.

      Warning:  HRP Rat Anti-Mouse Ig, k Light Chain contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

      Hazard statements

      May cause an allergic skin reaction.

      Precautionary statements

      Wear protective gloves / eye protection.

      Wear protective clothing.

      Avoid breathing mist/vapours/spray.

      If skin irritation or rash occurs: Get medical advice/attention.

      IF ON SKIN: Wash with plenty of water.

      Dispose of contents/container in accordance with local/regional/national/international regulations.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. ProClin is a trademark of Rohm and Haas Company.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      559751 Rev. 9
      Antibody Details
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      187.1

      The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.

      559751 Rev. 9
      Format Details
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      HRP
      Horseradish Peroxidase (HRP) is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide resulting in a visible colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with HRP and used in applications such as i) ELISA that uses a plate reader to measure absorbance of the colored product, or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
      HRP
      559751 Rev.9
      Citations & References
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      View product citations for antibody "559751" on CiteAb

      Development References (1)

      1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
      559751 Rev. 9

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.