BD Pharmingen™ PE Rat Anti-Mouse CD268 (BAFF-R)
Clone 7H22-E16 (RUO)
Multicolor flow cytometric analysis of mouse CD268 (BAFF-R) expression.
Left Panel - Spleen. BALB/c splenic leucocytes were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880), and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot), or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Cat. No. 565783; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes.
Right Panel: Bone Marrow. BALB/c bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 and BD Horizon™ BUV395 Rat Anti-Mouse IgM (Cat. No. 564025) antibodies, and either PE Rat IgG1, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus IgM were derived from CD45R/B220-positive gated events with the forward and side light-scatter characteristics of viable bone marrow cells.
Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
Multicolor flow cytometric analysis of mouse CD268 (BAFF-R) expression.
Left Panel - Spleen. BALB/c splenic leucocytes were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880), and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot), or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Cat. No. 565783; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes.
Right Panel: Bone Marrow. BALB/c bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 and BD Horizon™ BUV395 Rat Anti-Mouse IgM (Cat. No. 564025) antibodies, and either PE Rat IgG1, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus IgM were derived from CD45R/B220-positive gated events with the forward and side light-scatter characteristics of viable bone marrow cells.
Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
The 7H22-E16 monoclonal antibody specifically binds to CD268, which is also known as, B cell-activating factor receptor (BAFF-R), or BAFF Receptor 3 (BR3). CD268 is a type III transmembrane protein that is likewise known as Tumor necrosis factor receptor superfamily member 13C (Tnfrsf13c). CD268/BAFF-R is a receptor for CD257 (also known as, BAFF, Blys, TALL-1, or THANK) and is expressed on B cells and a subset of activated/memory CD4+ T cells. B cells express two other BAFF receptors, CD267/TACI, or CD269/BCMA, at various times during their differentiation. CD268/BAFF-R expression starts at the transitional stage of B cell development and increases throughout B cell maturation. CD267/BAFF-R mediates most BAFF-dependent functions including B cell survival, the activation of B cell proliferation and immunoglobulin secretion, and the costimulation of T cells. Overexpression of BAFF in mice and humans is associated with autoimmunity, whereas CD268/BAFF-R deficiency may cause severe impairment in humoral responses.
Development References (3)
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Giltiay NV, Lu Y, Allman D, Jørgensen TN, Li X. The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation.. J Immunol. 2010; 185(1):99-109. (Clone-specific: Flow cytometry). View Reference
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Naradikian MS, Perate AR, Cancro MP. BAFF receptors and ligands create independent homeostatic niches for B cell subsets.. Curr Opin Immunol. 2015; 34:126-129. (Biology). View Reference
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Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.. J Immunol. 2004; 173(2):807-17. (Immunogen: Flow cytometry). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.