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Real Yellow/Real Blue Family Campaign

BD Horizon RealYellow™ and RealBlue™ Reagents

The superior alternative to PerCP and PE tandem dyes

 

Reduced Monocyte Background

Reduced Monocyte 
Background

Reduced Spillover

Intracellular Staining

reduced monocyte background

RealYellow and RealBlueReagents Offer Decreased Monocyte Background Compared to PE-Cy Tandems

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BD HorizonRB780 Reagents offer reduced background compared to PE-Cy7

RY775-Improved-nonspecific-Binding
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BD Horizon™ RY775 Reagents provide improved nonspecific binding

reduced spillover

RealYellow and RealBlue Reagents Offer Reduced Spillover Compared to PE and PerCP Tandems

RB610 Reduced Spillover Compared to PE Dazzle and PE CF594
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BD Horizon RY610 Reagents show reduced spillover into the corresponding blue detectors.

RB705 Reduced Spillover Compared with PerCP and CY5.5
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BD Horizon RB705 Reagents show reduced spillover into the corresponding yellow-green detectors.

RY586 Reduced Spillover Compared with PE Tandem Dyes
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BD Horizon RY586 Reagents offer high resolution with minimal cross-laser excitation. Data was acquired on a BDFACSymphony A5 SE Cell Analyzer.

Intracellular marker detection

RealYellow and RealBlue Reagents Offer the Detection of Low-expression Surface and Intracellular Markers.

RY703-Reagent-Lower-Expression-Intracellular-Markers
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BD Horizon RY703 Reagent resolves low-expression intracellular markers

Cytokine-Marker
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BD Horizon RB780 Reagents support the detection of low-expression intracellular markers.

RY775-Resolves-Intracellular-Markers
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BD Horizon RY775 Reagent resolves intracellular markers

Swap Your Fluorochromes for the Cleaner Versions

FormatSpectralConventionalRelative BrightnessSpillover
(1-low, 4-high)
Alternative ToProducts
Blue Laser
RB545 1(Unique position)View
RB613 2PE-CF594, PE-Dazzle 594, BB630-P2, StarBright Blue 615View
RB670 2PE-Cy5, BB660-P2, StarBright Blue 675View
RB705 2PerCP-Cy5.5, BB700, StarBright Blue 700View
RB744 1BB755-PView
RB780 1PE-Cy7, BB790-PView
RB824 1PerCP/Fire™ 806, StarBrightBlue 810View
Yellow-Green Laser
RY586 1PEView
RY610 1PE-CF594, PE-Dazzle 594View
RY655 3PE-Cy5, StarBright Yellow 665View
RY703 2PE-Cy5.5, StarBright Yellow 720View
RY743 2PE/Fire 744View
RY775 2PE-Cy7, StarBright Yellow 800View

Note: PE and PE tandem dyes are excited by both the blue and yellow-green lasers. Be sure to check your instrument configuration.

 

Visit the BD Horizon Real Fluorochromes page to learn more about these fluorochromes and others in the family.

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