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BD Transduction Laboratories™ Purified Mouse Anti-Rat EAAT2
Clone 20/EAAT2 (RUO)
Western blot analysis of EAAT2 on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- rat EAAT2 antibody.
Western blot analysis of EAAT2 on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- rat EAAT2 antibody.
Product Details
BD Transduction Laboratories™
Excitatory amino acid transporter 2; Glutamate transporter 1; GLT1
Rat (QC Testing)
Mouse IgG1
Human EAAT2 aa. 562-574
Western blot (Routinely Tested)
66 kDa
250 µg/ml
AB_399172
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611654 Rev. 1
Antibody Details
20/EAAT2
Electrogenic-coupled (Na+ and K+) glutamate transporters in nerve terminals and glial cells are essential for maintaining subtoxic levels of extracellular excitatory amino acid neurotransmitters (e.g. glutamate and aspartate). They are also important for modifying synaptic transmission through the cotransport of ions and neurotransmitters. Excitatory amino acid transporter 2 (EAAT2) has 8 α-helical transmembrane domains and two reentrant pore-loop-like structures whose conformation is altered during ion neurotransmitter transport. EAAT2 is primarily expressed in astrocytes in adult brain and spinal cord, however it is also found in axons during fetal development. Mice deficient in EAAT2 exhibit lethal epileptic seizures with destruction of hippocampal neurons and an increased susceptibility to cold-induced cortical injury. Such defects in glutamine transporters have also been implicated in stroke, brain trauma, Alzheimer's disease, amyotrophic lateral sclerosis, and Huntington's disease. Thus, glutamine transporters, such as EAAT2, are critical for prevention of neurotoxic brain injury and may regulate glutamatergic synaptic transmission.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
611654 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611654 Rev.1
Citations & References
View product citations for antibody "611654" on CiteAb
Development References (4)
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Grunewald M, Kanner BI. The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate. J Biol Chem. 2000; 275(13):9684-9689. (Biology). View Reference
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Kirschner MA, Copeland NG, Gilbert DJ, Jenkins NA, Amara SG. Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2. Genomics. 1994; 24(2):218-224. (Biology). View Reference
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Tanaka K, Watase K, Manabe T, et al. Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1. Science. 1997; 276(5319):1699-1702. (Biology). View Reference
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Yamada K, Watanabe M, Shibata T, Nagashima M, Tanaka K, Inoue Y. Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression. J Neurosci. 1998; 18(15):5706-5713. (Biology). View Reference
611654 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.