Purified Mouse anti-PDPK1 (pS241)

BD Pharmingen™ Purified Mouse anti-PDPK1 (pS241)

Name: Purified Mouse anti-PDPK1 (pS241)
Brand: BD Pharmingen™
SKU: 558395

Clone J66-653.44.22

(RUO)

Western blot analysis of PDPK1 (pS241) in human T lymphocytes. Lysates from calyculin A- plus okadaic acid -treated Jurkat cells were probed with purified mouse anti-PDPK1 (pS241) at concentrations of 0.5 (lanes 1 and 4), 0.25 (lanes 2 and 5), and 0.125 µg/ml (lanes 3 and 6) with (lanes 1-3) or without (lanes 4-6) lambda protein phosphatase treatment. PDPK1 (pS241) is identified as a strong band of 63 kDa in the lysate without phosphatase treatment.

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Product Details

Brand: BD Pharmingen™

Alternative Name: PDK1 (pS241), PKB Kinase (pS241)

Reactivity: Human (QC Testing), Mouse,Rat (Predicted)

Isotype: Mouse IgG1, κ

Immunogen: Phosphorylated Human PDPK1 Peptide

Application: Western blot (Routinely Tested)

Target Molecular Weight: 63 kDa

Concentration: 0.5 mg/ml

RRID: AB_647291

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

558395 Rev. 2

Antibody Details

J66-653.44.22

The serine/threonine kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDPK1, also known as PDK1) contributes to the activation of many important kinases in the insulin and IGF-1 signaling pathways. It acts downstream of phosphatidylinositol 3-kinase (PI3-kinase) to phosphorylate residues in the activation loops of many cellular kinases, including protein kinase B (PKB/Akt), PKC isoforms, p70 S6 kinase, and PDPK1 itself. The autophosphorylation of PDPK1 at serine 241 (S241) has recently been suggested to play a role in the regulation of PDPK1. It has been proposed that PDPK1 activity plays a key role in the regulation of various cellular events such as cell proliferation, differentiation, and apoptosis.

The J666-653.44.22 monoclonal antibody recognizes the phosphorylated S241 in the activation loop of human PDPK1. The orthologous phosphorylation site in mouse and rat PDPK1 is S244.

558395 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

558395 Rev.2

Citations & References

Development References (2)

Komander D, Kular G, Deak M, Alessi DR, van Aalten DM. Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding. J Biol Chem. 2005; 280(19):18797-18802. (Biology). View Reference
Wick MJ, Ramos FJ, Chen H, et al. Mouse 3-phosphoinositide-dependent protein kinase-1 undergoes dimerization and trans-phosphorylation in the activation loop. J Biol Chem. 2003; 278(44):42913-42919. (Biology). View Reference

558395 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.