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BD Transduction Laboratories™ Purified Mouse Anti-Jun
Clone 3/Jun (RUO)
Western blot analysis of Jun on human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Jun antibody.
Immunohistochemical staining of Rabbit Muscle, SDS-treated, formalin-fixed, paraffin-embedded tissue section.
Immunohistochemical staining on rat brain. Formalin-fixed, paraffin-embedded section with citrate buffer pretreatment. 40X.
Western blot analysis of Jun on human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Jun antibody.
Immunohistochemical staining of Rabbit Muscle, SDS-treated, formalin-fixed, paraffin-embedded tissue section.
Immunohistochemical staining on rat brain. Formalin-fixed, paraffin-embedded section with citrate buffer pretreatment. 40X.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Mouse c-Jun aa. 26-175
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
39 kDa
250 µg/ml
AB_397717
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610326 Rev. 1
Antibody Details
3/Jun
The activator protein transcription factor (AP-1) was identified as a protein that recognizes specific sequences in the cis-control regions of the SV40 virus and the human metallothionein IIA gene. AP-1 is composed of protein products of two different gene families: jun and fos. The AP-1 transcription factor is either a homodimer of Jun proteins or a heterodimer of Jun and Fos proteins. The transcriptional activity of Jun is enhanced by phosphorylation in its activation domain at Ser63 and Ser73. Phosphorylation at both sites is necessary for stimulating the activating function of Jun. Jun is phosphorylated by JNK protein kinases that are activated by the same signals that potentiate Jun activity.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
610326 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610326 Rev.1
Citations & References
View product citations for antibody "610326" on CiteAb
Development References (5)
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Chang YC, Nakajima H, Illenye S, et al. Caspase-dependent apoptosis by ectopic expression of E2F-4. Oncogene. 2000; 19(41):4713-4720. (Clone-specific: Western blot). View Reference
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Doray B, Ghosh P, Griffith J, Geuze HJ, Kornfeld S. Cooperation of GGAs and AP-1 in packaging MPRs at the trans-Golgi network. Science. 2002; 297(5587):1700-1703. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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He T, Stepulak A, Holmstrom TH, Omary MB, Eriksson JE. The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J Biol Chem. 2002; 277(13):10767-10774. (Clone-specific: Western blot). View Reference
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Lamph WW, Wamsley P, Sassone-Corsi P, Verma IM. Induction of proto-oncogene JUN/AP-1 by serum and TPA. Nature. 1988; 334(6183):629-631. (Biology). View Reference
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Taupin D, Wu DC, Jeon WK, Devaney K, Wang TC, Podolsky DK. The trefoil gene family are coordinately expressed immediate-early genes: EGF receptor- and MAP kinase-dependent interregulation. J Clin Invest. 1999; 103(9):R31-R38. (Clone-specific: Immunoprecipitation). View Reference
610326 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.