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BD Transduction Laboratories™ Purified Mouse Anti-Caveolin 3
Clone 26/Caveolin 3 (RUO)
Western blot analysis of Caveolin 3 on rat muscle. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Caveolin 3.
Immunofluroescent staining of Rabbit Muscle with anti-Caveolin 3.
Western blot analysis of Caveolin 3 on rat muscle. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Caveolin 3.
Immunofluroescent staining of Rabbit Muscle with anti-Caveolin 3.
Product Details
BD Transduction Laboratories™
Rat (QC Testing), Mouse, Rabbit (Tested in Development)
Mouse IgG1
Rat Caveolin 3 aa. 3-24
Western blot (Routinely Tested), Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
18 kDa
250 µg/ml
AB_397801
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610420 Rev. 1
Antibody Details
26/Caveolin 3
Identified as a tyrosine phosphorylated protein in Rous sarcoma virus-transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN), and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin is part of a complex containing glycosylphosphatidylinositol (GPI)-linked molecules and cytoplasmic signaling proteins. Caveolin is a transmembrane adaptor molecule that can simultaneously recognize GPI-linked proteins and interact with downstream cytoplasmic signaling molecules, such as c-yes, Annexin II, and hetero-trimeric G proteins. Caveolin 3 has been identified as a distinct isoform which is expressed only in smooth, skeletal, and cardiac muscle.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
610420 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610420 Rev.1
Citations & References
View product citations for antibody "610420" on CiteAb
Development References (5)
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Lavitrano M, Bacci ML, Forni M, et al. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proc Natl Acad Sci U S A. 2002; 99(22):14230-14235. (Clone-specific: Western blot). View Reference
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Rybin VO, Xu X, Lisanti MP, Steinberg SF. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 2000; 275(52):41447-41457. (Clone-specific: Electron microscopy, Immunoprecipitation, Western blot). View Reference
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Song KS, Scherer PE, Tang Z, et al. Expression of caveolin-3 in skeletal, cardiac, and smooth muscle cells. Caveolin-3 is a component of the sarcolemma and co-fractionates with dystrophin and dystrophin-associated glycoproteins. J Biol Chem. 1996; 271(25):15160-15165. (Biology). View Reference
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Woodman SE, Park DS, Cohen AW, et al. Caveolin-3 knock-out mice develop a progressive cardiomyopathy and show hyperactivation of the p42/44 MAPK cascade. J Biol Chem. 2002; 277(41):38988-38997. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Zhao YY, Liu Y, Stan RV, Fan L, et al. Defects in caveolin-1 cause dilated cardiomyopathy and pulmonary hypertension in knockout mice. Proc Natl Acad Sci U S A. 2002; 99(17):11375-11380. (Clone-specific: Immunohistochemistry, Western blot). View Reference
610420 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.