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For Professionals in Research

Uncover up to 30 immune markers in a single experiment

The BD® AbSeq Immune Discovery Panel (IDP) is developed using our antibody-oligo based technology and consists of 30 different specificities against most major human immune markers lyophilized in a single tube. The BD® AbSeq IDP is tested to detect human immune markers and is designed to work on the BD Rhapsody™ Single-Cell Analysis System with BD Rhapsody™ Single-Cell RNA Assays and BD® Multiplexing Kits.
 

Find more information from the BD® AbSeq Immune Discovery Panel brochure.

 
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List of BD® AbSeq IDP Specificities

30 pre-titrated antibodies

 
Specificity Clone Oligo ID
CD3 UCHT1
AHS0231
CD4 SK3 AHS0032
CD8 SK1 AHS0228
CD11c
B-Ly6
AHS0056
CD14
MPHIP9
AHS0037
CD16
3G8 AHS0053
CD19
SJ25C1
AHS0030
CD25
2A3
AHS0026
CD27 M-T271 AHS0025
CD28 L293 AHS0138
 
Specificity Clone Oligo ID
CD45RA
HI100
AHS0009
CD56
NCAM16
AHS0019
CD62L
DREG-56
AHS0049
CD127
HIL-7R-M21
AHS0028
CD134
ACT35
AHS0013
CD137
4B4-1
AHS0003
CD161
HP-3G10
AHS0205
CD183 (CXCR3)
1C6/CXCR3
AHS0031
CD185 (CXCR5) RF8B2 AHS0039
CD186 (CXCR6) 13B 1E5 AHS0148
 
Specificity Clone Oligo ID
CD196 (CCR6) 11A9 AHS0034
CD197 (CCR7) 2-L1-A AHS0273
CD272 J168-540
AHS0052
CD278 DX29
AHS0012
CD279 EH12.1 AHS0014
CD357 (GITR) V27-580
AHS0104
CD366 (TIM-3) 7D3
AHS0016
HLA-DR G46-6
AHS0035
IgD IA6-2 AHS0058
IdM G20-127 AHS0198

BD® AbSeq IDP Protocol

Follow these simple steps to use the BD® AbSeq IDP:
 

Steps for reconstituting the lyophilized IDP and staining cells
 

  1. Remove the IDP tube from the foil bag and bring up to room temperature for 5 minutes
  2. Make sure the pellet is located at the bottom of the tube
  3. Add 35 µL of nuclease-free water to the bottom of the tube and allow antibodies to reconstitute for 5 minutes at room temperature
  4. Store the reconstituted antibodies on ice until the cells are ready for staining
    Note: Reconstituted antibodies should be used within 24 hours.
  5. To make 2X AbSeq labeling master mix, add 65 µL of BD Pharmingen™ Stain Buffer to the solution to bring it to a total of 100 µL
  6. Mix with 100 µL cell suspension prepared following Single Cell Labeling with BD® AbSeq Ab-Oligos (Doc ID 214394 Rev. 1.0) and stain the cells on ice for 30 minutes
  7. Add 3 mL BD Pharmingen™ Stain Buffer (FBS) to labeled cells and pipette-mix
  8. Centrifuge each tube at 400 x g for 5 minutes
  9. Uncap each tube and invert to decant supernatant into biohazardous waste. Keep the tube inverted and gently blot on a lint-free wipe to remove residual supernatant from tube rim
  10. Repeat steps 7–9 twice more for a total of three washes
  11. Resuspend pellet in 620 µL cold BD Pharmingen™ Stain Buffer (FBS) from the BD Rhapsody™ Cartridge Reagent Kit and proceed to single-cell capture
     

See the Single Cell Analysis Workflow with BD Rhapsody™ Systems.

PEFORMANCE

RESOURCES

 

To request a quote or place an order, email bdb_custom_orders@bd.com or contact your local BD sales representative.

 

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.