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Whole Transcriptome Analysis (WTA) Amplification Kit – 4 pack

Whole Transcriptome Analysis (WTA) Amplification Kit – 4 pack

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Product Details
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BD Rhapsody™
Single cell multiomics experiments on the BD Rhapsody™ platform
RUO


Description

The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification kit enables unbiased 3’-based capture, amplification and detection of transcriptomes at a single cell level. The BD Rhapsody WTA Amplification kit has been thoroughly tested and validated to generate consistent, high-quality whole transcriptome data across different workflows, different users and a wide range of cell inputs. The BD Rhapsody WTA Amplification kit allows you to identify genes of interest that can be used for building your targeted panels. Customizable targeted panel creation makes it easy to focus on the genes that really matter to your research. With the BD Single Cell Multiplexing kit (SMK) and the Rhapsody WTA Amplification kit working seamlessly together, you can save time and money by running multiple samples at once, giving you the option of multiplexing without compromising the quality of your data. With the BD Rhapsody WTA Amplification kit the power to uncover the whole transcriptome of a single cell is at your fingertips.This catalog number covers all the 4 kits needed for your single cell WTA experiment and includes BD Rhapsody™ Cartridge Reagent Kit (cat no. 633731); BD Rhapsody™ Cartridge Kit (cat no. 633733); BD Rhapsody™ cDNA Kit (cat no. 633773); BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit (cat no. 633801)


The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification kit enables unbiased 3’-based capture, amplification and detection of transcriptomes at a single cell level. The BD Rhapsody WTA Amplification kit has been thoroughly tested and validated to generate consistent, high-quality whole transcriptome data across different workflows, different users and a wide range of cell inputs. The BD Rhapsody WTA Amplification kit allows you to identify genes of interest that can be used for building your targeted panels. Customizable targeted panel creation makes it easy to focus on the genes that really matter to your research. With the BD Single Cell Multiplexing kit (SMK) and the Rhapsody WTA Amplification kit working seamlessly together, you can save time and money by running multiple samples at once, giving you the option of multiplexing without compromising the quality of your data. With the BD Rhapsody WTA Amplification kit the power to uncover the whole transcriptome of a single cell is at your fingertips.This catalog number covers all the 4 kits needed for your single cell WTA experiment and includes BD Rhapsody™ Cartridge Reagent Kit (cat no. 633731); BD Rhapsody™ Cartridge Kit (cat no. 633733); BD Rhapsody™ cDNA Kit (cat no. 633773); BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit (cat no. 633801)

Preparation And Storage

Store the BD Rhapsody™ Whole Transcriptome Analysis Amplification Kit stored at –20°C and BD Rhapsody™ cDNA Kit at –25°C to –15°C. Store the BD Rhapsody™ Cartridge Reagent Kit 2°C to 8°C and the BD Rhapsody™ Cartridge Kit at room temperature (15°C to 25°C ).

633802 Rev. 1
Components
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Description Quantity/Size Part Number
Cartridge Reagent Kit 1 Each (1 ea) 633731
Cartridge Kit 1 Each (1 ea) 633733
cDNA Kit 1 Each (1 ea) 633773
Whole Transcriptome Analysis (WTA) Amplification Kit 1 Each (1 ea) 633801
633802 Rev. 1
Citations & References
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Development References (10)

  1. Li H, Wang H, Sokulsky L, et al. Single-cell transcriptomic analysis reveals key immune cell phenotypes in the lungs of patients with asthma exacerbation. J Allergy Clin Immunol. 2020; S0091(20):31400-7. (Biology). View Reference
  2. Li Z, Li M, Shi SX, et al. Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. J Exp Med. 2020; 217(12):e20200213. (Biology). View Reference
  3. Schulte-Schrepping J, Reusch N, Paclik D, et al. Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment. Cell. 2020; 182(6):1419-1440. (Biology). View Reference
  4. Song B, Xiong G, Luo H, Zuo Z, Zhou Z, Chang X. Single-cell RNA sequencing of mouse neural stem cell differentiation reveals adverse effects of cadmium on neurogenesis. Food Chem Toxicol. 2021; (148):111936. (Biology). View Reference
  5. Song L, Zhang S, Yu S, et al. Cellular heterogeneity landscape in laryngeal squamous cell carcinoma. Int J Cancer. Int J Cancer. 2020; 147(10):2879-2890. (Biology). View Reference
  6. Wen M, Cai G, Ye J, Liu X, Ding H, Zeng H. Single-cell transcriptomics reveals the alteration of peripheral blood mononuclear cells driven by sepsis. Ann Transl Med. 2020; 8(4):125. (Biology). View Reference
  7. Wu B, Wang W, Wang H, et al. Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2. Front Oncol. 2020; (10):566599. (Biology). View Reference
  8. Wu M, Wang C, Shi B, et al. A novel recombinant cccDNA-based mouse model with long term maintenance of rcccDNA and antigenemia. Antiviral Res. 2020; 180:104826. (Biology). View Reference
  9. Xie X, Shi Q, Wu P, et al. Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection.. Nat Immunol. 2020; 21(9):1119-1133. (Biology). View Reference
  10. Zhao L, Yao C, Xing X, et al. Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells. Nat Commun. 2020; 11(1):5683. (Biology). View Reference
View All (10) View Less
633802 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.