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BD Pharmingen™ Purified Mouse Anti-PMS2
Clone A16-4 (RUO)
Western blot analysis of PMS2. Lysate from A431 human epidermal cells was probed with anti-PMS2 (clone A16-4, Cat. No. 556415) between 2.0 and 0.08 µg/ml and identifies PMS2 at ~100 kDa.
Western blot analysis of PMS2. Lysate from A431 human epidermal cells was probed with anti-PMS2 (clone A16-4, Cat. No. 556415) between 2.0 and 0.08 µg/ml and identifies PMS2 at ~100 kDa.
Product Details
BD Pharmingen™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Recombinant Human PMS2
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Reported)
100 kDa
0.5 mg/ml
AB_396410
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556415 Rev. 1
Antibody Details
A16-4
The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In humans the proteins are called MutS homolog2 (MSH2), MutL homolog (MLH1), and PMS2 which is also a homolog of MutL. After MSH2 and a partner bind to a mismatched DNA duplex, the complex is joined by a heterodimer of MLH1 and PMS2 which together help facilitate the later steps in mismatch repair. Two other members of this family, MSH3 and MSH6, can also join the MSH2-containing complex to help facilitate repair. The reduced molecular weight of PMS2 is ~100 kDa. mAb A16-4 recognizes human and mouse PMS2. Recombinant human PMS2 (C-terminal half) was used as immunogen.
556415 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556415 Rev.1
Citations & References
View product citations for antibody "556415" on CiteAb
Development References (5)
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Cleaver JE. It was a very good year for DNA repair. Cell. 1994; 76(1):1-4. (Biology). View Reference
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Marsischky GT, Filosi N, Kane MF, Kolodner R. Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair. Genes Dev. 1996; 10(4):407-420. (Biology). View Reference
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Prolla TA, Christie DM, Liskay RM. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene. Mol Cell Biol. 1994; 14(1):407-415. (Biology). View Reference
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Prolla TA, Pang Q, Alani E, Kolodner RD, Liskay RM. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science. 1994; 265(5175):1091-1093. (Biology). View Reference
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Su SS, Modrich P. Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc Natl Acad Sci U S A. 1986; 83(14):5057-5061. (Biology). View Reference
556415 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.