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Purified Mouse Anti-KIF3A
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human,Mouse (Tested in Development)
Mouse IgG1
Human KIF3A aa. 563-671
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80/85 kDa
250 µg/ml
AB_398968
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611508 Rev. 2
Antibody Details
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28/KIF3A

The ability of the kinesin superfamily of motor proteins to hydrolyze ATP as they move progressively along microtubules is important for organelle transport and cell division. Kinesins are grouped according to the location of the motor domain in the N-terminal, middle, or C-terminal region of the protein. A family of N-terminal motor domain kinesin proteins includes KIF3A/3B, KRP85/95, and Klp68d/64. These proteins form heterodimeric two-headed motors that interact with kinesin associated protein (KAP). The KIF3A/3B/KAP3 heterotrimeric motor has plus-end directed microtubule sliding activity. This heterotrimeric motor is expressed ubiquitously and is used for anterograde transport of membranous organelles. KIF3A- and KIF3B-deficient mice have defects in the formation of the cilia located in the embryonic node. In addition, KIF3A localizes to the basal body of the connecting cilium axoneme and to vesicles at the pre-synaptic ribbon in photoreceptor cells. Thus, KIF3 motors are involved in microtubule-based formation and movement of cilia and the transport of synaptic vesicles.

This antibody is routinely tested by the Western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611508 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611508 Rev.2
Citations & References
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View product citations for antibody "611508" on CiteAb

Development References (3)

  1. Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 1998; 279(5350):519-526. (Biology). View Reference
  2. Marszalek JR, Ruiz-Lozano P, Roberts E, Chien KR, Goldstein LS. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II.. Proc Natl Acad Sci USA. 1999; 96(9):5043-8. (Biology). View Reference
  3. Muresan V, Lyass A, Schnapp BJ. The kinesin motor KIF3A is a component of the presynaptic ribbon in vertebrate photoreceptors. Neuroscience. 1999; 19(3):1027-1037. (Biology). View Reference
611508 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.