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Western blot analysis of KIF3A on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of KIF3A.
Rat Neurons
BD Transduction Laboratories™ Purified Mouse Anti-KIF3A
BD Transduction Laboratories™ Purified Mouse Anti-KIF3A
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The ability of the kinesin superfamily of motor proteins to hydrolyze ATP as they move progressively along microtubules is important for organelle transport and cell division. Kinesins are grouped according to the location of the motor domain in the N-terminal, middle, or C-terminal region of the protein. A family of N-terminal motor domain kinesin proteins includes KIF3A/3B, KRP85/95, and Klp68d/64. These proteins form heterodimeric two-headed motors that interact with kinesin associated protein (KAP). The KIF3A/3B/KAP3 heterotrimeric motor has plus-end directed microtubule sliding activity. This heterotrimeric motor is expressed ubiquitously and is used for anterograde transport of membranous organelles. KIF3A- and KIF3B-deficient mice have defects in the formation of the cilia located in the embryonic node. In addition, KIF3A localizes to the basal body of the connecting cilium axoneme and to vesicles at the pre-synaptic ribbon in photoreceptor cells. Thus, KIF3 motors are involved in microtubule-based formation and movement of cilia and the transport of synaptic vesicles.
This antibody is routinely tested by the Western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 1998; 279(5350):519-526. (Biology). View Reference
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Marszalek JR, Ruiz-Lozano P, Roberts E, Chien KR, Goldstein LS. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II.. Proc Natl Acad Sci USA. 1999; 96(9):5043-8. (Biology). View Reference
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Muresan V, Lyass A, Schnapp BJ. The kinesin motor KIF3A is a component of the presynaptic ribbon in vertebrate photoreceptors. Neuroscience. 1999; 19(3):1027-1037. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.