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Purified Mouse Anti- HNF-1β
Purified Mouse Anti- HNF-1β
Western blot analysis of HNF-1β on a rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- HNF-1β antibody.
Purified Mouse Anti- HNF-1β

Immunofluorescence staining of normal rat kidney.

Western blot analysis of HNF-1β on a rat kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- HNF-1β antibody.

Immunofluorescence staining of normal rat kidney.

Product Details
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BD Transduction Laboratories™
Hepatocyte Nuclear Factor-1β
Rat (QC Testing), Human, Mouse, Dog (Tested in Development)
Mouse IgG1
Mouse HNF-1β aa. 1-101
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
51 kDa
250 µg/ml
AB_399805
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612504 Rev. 1
Antibody Details
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25/HNF-1β

HNF's (Hepatocyte Nuclear Factor) are POU-homeodomain-containing liver enriched transcription factors that include HNF1-α, HNF1-β, HNF-3α, HNF-3β, HNF-4 and HNF-6. HNF-1 is expressed in liver, kidney, and intestine, while HNF-1β is expressed highest in kidney with lower levels in intestine, lung, and liver. Both HNF-1 and HNF-1β have short N-terminal α-helical segments that facilitate homodimerization (A-domain), a region related to POU-specific A-box (B-domain), and a homeodomain (C-domain). The HNF-1 and HNF-1β homo- and hetero-dimers transactivate genes required for development of various tissues. Both transcription factors can bind the consensus HNF1 inverted palindromic sequence GTTAATNATTAAC. HNF-1 represses its own transcription and data suggests that there is coordinate regulation between HNF-1 and HNF-4. Although HNF-4 induces HNF-1 transcription, HNF-1 downregulates HNF-4 activity by binding to the AF2 region in the activation domain of HNF-4. Thus, HNF transcription factors may be involved in various aspects of the regulation of gene transcription throughout development.

612504 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612504 Rev.1
Citations & References
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Development References (3)

  1. De Simone V, De Magistris L, Lazzaro D, et al. LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia. EMBO J. 1991; 10(6):1435-1443. (Biology). View Reference
  2. Ringeisen F, Rey-Campos J, Yaniv M. The transactivation potential of variant hepatocyte nuclear factor 1 is modified by alternative splicing. J Biol Chem. 1993; 268(34):25706-25711. (Biology). View Reference
  3. Soubt MK, Marksitzer R, Menoud PA, Nagamine Y. Role of tissue-specific transcription factor LFB3 in a cyclic AMP-responsive enhancer of the urokinase-type plasminogen activator gene in LLC-PK1 cells. Mol Cell Biol. 1998; 18(8):4698-4706. (Biology). View Reference
612504 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.