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Purified Mouse Anti-AKAP149
Purified Mouse Anti-AKAP149
Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.
Purified Mouse Anti-AKAP149

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of AKAP149 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- AKAP149 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Rat (Tested in Development)
Mouse IgG1
Human AKAP149 aa. 66-212
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
149 kDa
250 µg/ml
AB_398043
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610720 Rev. 1
Antibody Details
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6/AKAP149

The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610720 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610720 Rev.1
Citations & References
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Development References (5)

  1. Dell'Acqua ML, Scott JD. Protein kinase A anchoring. J Biol Chem. 1997; 272(20):12881-12884. (Biology). View Reference
  2. Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  3. Steen RL, Collas P. Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression. J Cell Biol. 2001; 153(3):621-626. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A. A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta. J Biol Chem. 2002; 277(40):36955-36961. (Biology: Immunoprecipitation, Western blot). View Reference
  5. Trendelenburg G, Hummel M, Riecken EO, Hanski C. Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain. Biochem Biophys Res Commun. 1996; 225(1):313-319. (Biology). View Reference
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610720 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.