Preparation And Storage
Recommended Assay Procedures
ELISA Capture: The AN-18 antibody has been reported to be useful in a sandwich ELISA that measures mouse IFN-γ protein levels. The purified AN-18 antibody (Cat. No. 551309) can be used as a capture antibody when paired with biotinylated R4-6A2 rat anti-mouse IFN-γ antibody (Cat. No. 551506) as the detecting antibody and with recombinant mouse IFN-γ (Cat. No. 554587) as the standard. The purified AN-18 antibody should be titrated 1-4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IFN-γ ranging from 500 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our website, www.bdbiosciences.com.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IFN-γ in serum or plasma our mouse IFN-γ OptEIA™ Set (Cat. No. 551866) or OptEIA Kit (Cat. No. 550582) are specially formulated and recommended.
Immunoprecipitation: The AN-18 antibody has been reported to be used for the immunoprecipitation of mouse IFN-γ; however, the antibody is not routinely tested for this application at Pharmingen.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The AN-18 antibody reacts with the mouse interferon-γ (IFN-γ) protein but does not recognize human IFN-γ (reactivity with other species has not been established). AN-18 has been reported to be useful for the neutralization of the antiviral and macrophage-activating activities of IFN-γ. The immunogen used to generate this hybridoma was mouse IFN-γ protein that was secreted by a T cell lymphoma cell line.
This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
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Prat M, Gribaudo G, Comoglio PM, Cavallo G, Landolfo S. Monoclonal antibodies against murine gamma interferon. Proc Natl Acad Sci U S A. 1984; 81(14):4515-4519. (Immunogen: Immunoprecipitation). View Reference
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Slade SJ, Langhorne J. Production of interferon-gamma during infection of mice with Plasmodium chabaudi chabaudi. Immunobiology. 1989; 179(4-5):353-365. (Clone-specific: ELISA, Neutralization). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.